Sphere Fluidics
Booth Number 2744
January 25-29, 2025
San Diego, CA, USA
Join us at SLAS2025, where curiosity meets innovation. Discover the latest in laboratory automation, omics technologies, and biomarker diagnostics. Engage with leaders and innovators as we explore cutting-edge solutions for advancing scientific discovery and technology under one roof. Don’t miss out on this opportunity to see where your curiosity can take you!
Insights and Innovations
Join Sphere Fluidics at the Show
Solutions Spotlight with Sphere Fluidics
Solutions Spotlight Theater (Booth #2907)
Title: Meet Cyto-Mine® Chroma: the next-generation platform for functional characterization and selection of live single cells.
Tuesday January 28, 2025
12:00 PM – 12:20 PM
Richard Hammond, CTO, Sphere Fluidics
Poster Presentation by John Lesnick
Cyto-Mine® Chroma: Accelerating Single-Cell Analysis and Sorting using Multi-Laser Picodroplet Technology
Exhibit Hall
Poster Number: 1177-B
Poster Presentations: Session B
Track Name: Automation Technologies
Monday January 27, 2025
2:00 PM – 3:00 PM
John Lesnick, Senior Field Applications Scientist, Sphere Fluidics
POSTER PRESENTATION DETAILS
Antibody therapeutics dominate drug development; however, lengthy workflows and rising costs in their discovery and development present significant challenges. Sphere Fluidics’ Cyto-Mine® system leverages picodroplet technology, microfluidics, and imaging to overcome these hurdles in Antibody Discovery (AbD) and Cell Line Development (CLD), accelerating therapeutic development.
Here we introduce the next-generation Cyto-Mine® platform, Cyto-Mine® Chroma. This advanced microfluidic platform, equipped with multi-laser, single-cell analysis capabilities, enables high-throughput, multiplex functional screening of millions of cells in a single day.
Our findings demonstrate that Cyto-Mine® Chroma can precisely sort and dispense cells based on fluorescence signals and sequential gating. Additionally, it achieves over 90% accuracy in isolating cells based on productivity and membrane dye from mixed populations. In an experiment simulating an AbD workflow, Cyto-Mine® Chroma successfully isolated rare cells secreting antigen-specific antibodies with high precision.
Furthermore, by utilizing a viability dye alongside IgG-specific probes, we demonstrated its ability to isolate CHO cells producing human IgG4 based on both productivity and viability, without compromising cell performance. These results highlight Cyto-Mine® Chroma’s robust multiplexing capabilities, making it an invaluable tool for isolating specific cell populations in both CLD and AbD workflows.
