Discover the Versatility of Cyto-Cellect®Mouse in Detecting Mouse IgG

The Cyto-Cellect® Mouse from Sphere Fluidics is specifically designed to be used with the Cyto-Mine® Single Cell Analysis Platform.

This kit identifies whole-molecule mouse IgG secreted by cells encapsulated in picodroplets (Figure 1). Fluorescent probes in the kit bind to mouse IgG post-secretion from these encapsulated cells.

In combination with Cyto-Mine®, this kit offers a rapid assay to measure single-cell productivity by detecting secreted IgG, thereby facilitating the identification and selection of cells with the highest productivity

Key Highlights:

Compatibility with Cyto-Mine®

Cyto-Cellect® Mouse can be used in combination with Cyto-Mine® to allow sorting and dispensing of single cells, based on their productivity.

Short incubation time in Cyto-Mine®

Discrete FRET+ population is detectable as early as 60 minutes following incubation of cells and Cyto-Cellect® Mouse probes in Cyto-Mine®. Signal intensity increases upon longer incubation.

High Signal Intensity & Sensitivity

The kit shows good signal intensity in both plate reader as well as Cyto-Mine® assays, with more than 4-fold increase in FRET signal.

Low cross-reactivity

The kit offers no/low cross-reactivity with human IgG, mouse IgM, rabbit IgG and rat IgG.

Figure 1. Schematic diagram of the Cyto-Cellect® Mouse for IgG secreting cells. A customized pair of IgG-specific fluorescent probes are contained within each picodroplet. IgG secreted from the encapsulated cell is recognized by the detection probe pair forming a ternary FRET complex that induces a change in fluorescent signal.

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