Built on picodroplet technology
At the heart of our platforms are picodroplets – picolitre-volume aqueous droplets in a carrier oil. Each droplet acts as a tiny separate test tube. Cells are encapsulated in droplets with the assay reagents and passed through microfluidics for sorting and dispensing. Miniaturized, high-speed and fully automated to streamline your application.
Engineering cells individually
Picodroplets support your workflow
Cell engineering workflows can take time – often days to execute. Picodroplets remain stable for these extended periods and allow high rates of gas transfer to maintain long-term cell viability, up to 72 hours from encapsulation.
Picodroplets support either approach
We have implemented workflows for both transduction and chemical transfection. We demonstrated successful editing using baculovirus, with 35% of all cells showing outgrowth and 3% with successful editing events. We also demonstrated CRISPR-RNP editing using chemical transfection, with 20% of cells edited and 16% showing outgrowth.
A widely-applicable technology
Picodroplets work with multiple cell types: Murine neural stem cells, Suspension cells – CHO, Jurkat, THP1, RAJI, Adherent cells – HCT116, A431, A549, HEK293.
Engineer, characterize and select
Your entire workflow can be run within picodroplets. Engineer your cells, assay them to identify successful editing, select the good ones, and dispense them into plates for subsequent outgrowth. Automated, robust and easy to use to free up your time and mental capacity.