Gene editing in picodroplets
It’s not just sorting and dispensing. Picodroplets are ultra-miniature reaction vessels and can be used to engineer cells – individually. Talk to our applications support team to discuss how our technology could work for your projects.
- Engineering mammalian cells
- Transduction and transfection
- Effortless selection and sorting post-modification
Built on picodroplet technology
At the heart of our platforms are picodroplets – picolitre-volume aqueous droplets in a carrier oil. Each droplet acts as a tiny separate test tube. Cells are encapsulated in droplets with the assay reagents and passed through microfluidics for sorting and dispensing. Miniaturized, high-speed and fully automated to streamline your application.
Engineering cells individually
Picodroplets support your workflow
Cell engineering workflows can take time – often days to execute. Picodroplets remain stable for these extended periods and allow high rates of gas transfer to maintain long-term cell viability, up to 72 hours from encapsulation.
Transduction and transfection
Picodroplets support either approach
We have implemented workflows for both transduction and chemical transfection. We demonstrated successful editing using baculovirus, with 35% of all cells showing outgrowth and 3% with successful editing events. We also demonstrated CRISPR-RNP editing using chemical transfection, with 20% of cells edited and 16% showing outgrowth.
Validated on multiple cell types
A widely-applicable technology
Picodroplets work with multiple cell types: Murine neural stem cells, Suspension cells – CHO, Jurkat, THP1, RAJI, Adherent cells – HCT116, A431, A549, HEK293.
Engineer, characterize and select
Your entire workflow can be run within picodroplets. Engineer your cells, assay them to identify successful editing, select the good ones, and dispense them into plates for subsequent outgrowth. Automated, robust and easy to use to free up your time and mental capacity.
Seeing is believing
Don’t take our word for it – see our technology in action. We provide demos and proof-of-concept projects for our customers so you can build confidence in what our platforms can do for your cells.
- Demos at your site or at our site in Cambridge, UK
- Use our cell lines and assays, or use yours
- Proof-of-concept projects – try something novel
Resources & insights
What our customers are saying
“Single cell analysis allows detection of those very rare antibodies or potential drugs with unique properties that are exceptionally hard to find. My experience in drug discovery since the early 1990s all points to the importance of diversity, and Cyto-Mine® is well-placed to explore this effectively. This technology takes screening capability, traditionally conducted through plate-based analysis, to a whole new level via continuous flow. There is huge potential for the instrument in discovering the rarest cells and best antibodies”
Dr Tristan Vaughan
Antibody & Protein Engineer Executive
We’re the droplet microfluidic technology company for single cell analysis and our customers are at the center of what we do
is future proof
Our platform is built upon robust evidence from key opinion leaders
Our collaboration with customers is
second to none
Have a question for us?
Our team of experts are on hand to provide you with the information and support you need. Whether you have a question about our products, services or training, we're always happy to assist you