Discover a new picodroplet-based approach for the functional validation of CAR-T cells.
This application note presents an easy-to-use and robust method for studying cell-mediated cytotoxicity.
Engineered T cells and target cells are co-encapsulated in picolitre-volume aqueous droplets (picodroplets), along with granzyme B substrate. Granzyme B release by the T cell, a readout of the cells ability to recognize and kill the cancer cell, can be captured to determine the level of potential anti-cancer activity. In doing so, this method enables rapid cell product efficacy readouts to streamline the QC release of cell product.