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Date: May 20-24, 2023
Location: Palais des congrès de Montréal, Montréal, Québec, Canada
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Poster Presentation: 23rd May 2023, Hall 210

Alex McNeil Headshot

Whether you’re a seasoned single cell researcher or simply curious about the latest innovative methodologies and technologies, our poster at CYTO 2023 is a must-see! Don’t miss this opportunity to engage in stimulating discussions, exchange ideas, and expand your knowledge in the field.

Poster Title: Novel Bioassays in Picodroplets for Single-Cell Analysis

Poster Authors: Alex McNeil, Elena Shvets, Frank Gesellchen, Xin Liu

Poster Abstract: Many current technologies used in drug discovery and preclinical development programs typically involve multi-step operations, extensive hands-on time, and lengthy liquid handling processes as well as low-throughput bioassays for functional validation and verification. We will discuss how microfluidic devices, with well-defined microchannel geometries and accurate flow control, can be used to generate uniform picolitre volume droplets (picodroplets) for use as miniaturized “test tubes” for high-throughput bioassays.

Picodroplets can be used in a diverse range of applications. An important application of picodroplet microfluidics is single-cell analysis, in which single cells (and their corresponding secreted molecules) are encapsulated in a volume that is 100,000s times lower than a typical-bulk volume method. This not only enables the rapid detection of secreted molecules and high-throughput sample processing but also time- and cost-effective screening compared to conventional assays. More importantly, we have demonstrated that cells remain viable and functional in the picodroplets for several days, making the technology the ideal tool for a wide range of bioassays and research projects.

Picodroplet-based technology can isolate and screen various types of cells, including bacteria, yeast, Chinese hamster ovary cells, HEK cells, primary B and T cells, and even stem cells. We will demonstrate how novel picodroplet bioassays can be used in a wide range of applications including biotherapeutic discovery, cell line development, cell therapy research, quality control, genome engineering (comprising CRISPR techniques), and synthetic biology.

Poster Time and Location: Tuesday, May 23rd: 5:00-7:00 pm in Hall 210

Mark your calendar, and make sure to visit Alex McNeil at his poster. He’s looking forward to seeing you there!

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