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Date: May 28th
Location: Switzerland Innovation Park Basel Area, Hegenheimermattweg 167D, 4123 Allschwil
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Join us for the next-gen single-cell omics workshop to explore the cutting-edge of single-cell technologies and their applications in biomedicine.

Look out for our Presentation in the Lectures: Single-cell handling and analysis - May 28th 10:00 - 11:00 am

Richard Hammond will be presenting

Title: 

Picodroplets for cell line engineering: a novel automation approach

Description:

Droplet-based microfluidic systems have matured to become an important tool in biotechnology R+D for high-throughput experimentation. The partitioning of an aqueous phase into large numbers of separate droplets using an oil phase provides a robust, low cost way to assess individuals in a population.

Originally applied to genomics via techniques such as digital droplet PCR, droplets are now increasingly being used to assess cell heterogeneity via the proteome. In particular, at Sphere Fluidics, we use droplets in the picolitre volume range (hence ‘picodroplets’) and microfluidics to implement complex workflows for single cell characterization and population enrichment.

In this talk, we will introduce the core technologies that enable the application of picodroplets to cell engineering and how these are integrated into an easy-to-use commercial product. We will show how the approach is used in cell line development and present early data showing the use of droplets to enable gene editing on a cell-by-cell basis.

Date & Time:

May 28th, 10:00 am

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